EPH Receptor B4 antibody

Catalog No. ABIN1107116

The Rat Monoclonal anti-EPH Receptor B4 antibody has been validated for WB, IF, EIA and IHC (fro). It is suitable to detect EPH Receptor B4 in samples from Mouse.

Quick Overview for EPH Receptor B4 antibody (ABIN1107116)

Target: EPH Receptor B4 (EPHB4)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This EPH Receptor B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: VEB4-7E4

Product Details anti-EPH Receptor B4 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Purified

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for EPH Receptor B4

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EPHB4

Background: Erythropoietin-producing human hepatocellular carcinoma (Eph) receptors and ephrins are membrane proteins. They are classified into 2 broad subclasses, namely A and B, according to structural homologies and binding specificities. Eph receptors are tyrosine kinases, which autophosphorylate upon binding to their cognate ephrin ligands. Eph receptors and ephrins are frequently expressed in reciprocal patterns that correlate with cellular boundaries during embryonic development. The interaction between EphB4 and its ligand, Ephrin-B2, plays an important role in cell-cell, cell-extracellular matrix interactions as well as in cell migration, adhesion and proliferation. During the early stages of vascular development, EphB4 is specifically expressed in venous endothelium, whereas Ephrin-B2 is expressed in arterial endothelium. In mouse embryo's, EphB4 and Ephrin-B2 are essential for embryonic heart development and angiogenesis. In adult microcirculation, EphB4 is not a ubiquitous marker of arterial/venous polarity, but is expressed along both venules and arterioles. Furthermore, EphB4 is upregulated by endothelial cells along blind-ended capillary sprouts versus connecting capillaries. As such, EphB4 is thought to play a role in the patterning of new vessels during angiogenesis. EphB4 is also expressed in a variety of tumor cells, like gastrointestinal, prostate, bladder, breast, liver, lung and ovarian cancers, as well as leukemia, mesothelioma, malignant breast tumors and melanoma. Reduction of EphB4 activity accelerated tumorigenesis in colon and rectum. In head and neck squamous cell carcinoma and endometrial carcinoma, overexpression of EphB4 is inversely related to a poor prognosis. However, in mesothelioma, up-regulation of EphB4 resulted in growth of the tumor. Besides the essential expression of EphB4, coexpression of other EphB4 family members or EphB-ligands may affect tumor cell viability and proliferation as well.Synonyms: Ephrin type-B receptor 4, HTK, TYRO11, Tyrosine-protein kinase TYRO11, Tyrosine-protein kinase receptor HTK

Gene ID: 13846

NCBI Accession: NP_034274

UniProt: P54761

Pathways: RTK Signaling