Fibronectin antibody

Catalog No. ABIN1107236

The Mouse Monoclonal anti-Fibronectin antibody has been validated for WB, EIA and IHC (fro). It is suitable to detect Fibronectin in samples from Human, Rat, Chicken, Rabbit and Guinea Pig.

Quick Overview for Fibronectin antibody (ABIN1107236)

Target: Fibronectin

Reactivity: Human, Rat, Chicken, Rabbit, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DH1

Product Details anti-Fibronectin Antibody

Specificity: The antibody is specific to extradomain A (EDA) sequence of a Cellular Fibronectin and recognizes thus only the Cellular Fibronectin (cFN). Clone DHI has been shown to specifically block Chondrocyte condensation in Chicken embryos (ref. 5).

Cross-Reactivity (Details): Species reactivity (tested):Human, Chicken, Rat, Rabbit and Guinea Pig.

Purification: Ig Fraction

Immunogen: Purified Fibronectin from A8387 fibrosarcoma cells (ref. 21). Hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % (w/v) Sodium Azide, 1.0 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (AM32091PU-N Clone BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas.Synonyms: CIG, Cold-insoluble globulin, FN1

Gene ID: 2335

NCBI Accession: NP_002017

UniProt: P02751