Endothelin 1 antibody (C-Term)

Catalog No. ABIN110809

This Rat Monoclonal antibody specifically detects Endothelin 1 in RIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Endothelin 1 antibody (C-Term) (ABIN110809)

Target: Endothelin 1 (EDN1)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Endothelin 1 antibody is un-conjugated

Application: Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3G10

Product Details anti-Endothelin 1 Antibody

Binding Specificity: C-Term

Sequence: C-H-L-D-I-I-W

Specificity: Reacts with the C-terminal of endothelins 1, 2 and 3. 3G10 does not react with the endothelin precursor molecule, big endothelin.

Purification: Protein G affinity chromatography

Immunogen: Synthetic human endothelin-C terminal fragment, conjugated to BSA

Isotype: IgG2b

Application Details

Application Notes: RIA / IRMA. Immunohistochemistry on frozen sections: Frozen sections processed with dry acetone are recommended. Paraformaldehyde is notrecommended as it destroys the epitope.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: Endothelin-1

Background: Endothelins are potent vasoconstrictors and critical components in the progression of pulmonary arterial hypertension a devastating and rapidly progressive condition that leads to right ventricular strain and dysfunction.Synonyms: Big endothelin-1, EDN1, EDN2, ET-2, ET-3, Endothelin-2, Endothelin1, PPET1, PPET2, PPET3, Preproendothelin-1, Preproendothelin-2, Preproendothelin-3

Gene ID: 1906

NCBI Accession: NP_001946

UniProt: P05305

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size