M-CSF/CSF1 antibody

Catalog No. ABIN1108092

This anti-M-CSF/CSF1 antibody is a Rat Monoclonal antibody detecting M-CSF/CSF1 in FACS and IHC (fro). Suitable for Mouse.

Quick Overview for M-CSF/CSF1 antibody (ABIN1108092)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This M-CSF/CSF1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-MP58

Product Details anti-M-CSF/CSF1 Antibody

Specificity: The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells ut to the monocytic level, but also on grnaulocytes. ERxpression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commenly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hemopoietic islands in various organs, and for embryonal studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 and ER-MP-20.

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Protein G

Immunogen: Balb/c macrophage precursor cell hybrids

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at < -20 °C for at least 6 month.

Expiry Date: 6 months

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: Macrophage Colony Stimulating Factor (M-CSF)

Background: Synonyms: CSF-1, CSF1, MCSF, Macrophage colony-stimulating factor 1

Gene ID: 12977

NCBI Accession: NP_001107001

UniProt: P07141

Pathways: RTK Signaling