MBP/MBL antibody

Catalog No. ABIN1108115

The Rat Monoclonal anti-MBP/MBL antibody is suitable to detect MBP/MBL in samples from Mouse. It has been validated for WB, IHC (fro), EIA and IF.

Quick Overview for MBP/MBL antibody (ABIN1108115)

Target: MBP/MBL (Mannan Binding Protein/Mannan Binding Lectin (MBP/MBL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MBP/MBL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Clone: 14D12

Product Details anti-MBP/MBL Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Protein G

Immunogen: Purified MBL-C

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for MBP/MBL

Target: MBP/MBL (Mannan Binding Protein/Mannan Binding Lectin (MBP/MBL))

Alternative Name: Mannan Binding Protein

Background: Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL-associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A.Synonyms: MBL, MBL2, MBP, MBP-C, MBP1, Mannan-binding protein, Mannose-binding lectin, Mannose-binding protein C

Gene ID: 17195

NCBI Accession: NP_034906

UniProt: P41317