LY96 antibody

Catalog No. ABIN1108190

The Mouse Monoclonal anti-LY96 antibody is suitable to detect LY96 in samples from Human. It has been validated for WB, IF, EIA and Func.

Quick Overview for LY96 antibody (ABIN1108190)

Target: LY96 (Lymphocyte Antigen 96 (LY96))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY96 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: 4H1

Product Details anti-LY96 Antibody

Specificity: This antibody reacts with soluble MD-2 (sMD-2) and the sMD-2/Toll-like receptor 4 (TLR4, CD284) complex. It reacts with both the monomeric and the polymeric form of sMD-2.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G

Immunogen: Baculovirally expressed His-tagged human MD-2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for LY96

Target: LY96 (Lymphocyte Antigen 96 (LY96))

Alternative Name: MD-2

Background: TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.Synonyms: ESOP-1, ESOP1, LY96, Lymphocyte antigen 96, MD2

Gene ID: 23643

NCBI Accession: NP_001182726

UniProt: Q9Y6Y9

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades