Nitrotyrosine antibody

Catalog No. ABIN1108460

The Mouse Monoclonal anti-Nitrotyrosine antibody has been validated for WB, IHC (fro), IHC (p) and EIA. It is suitable to detect Nitrotyrosine in samples from .

Quick Overview for Nitrotyrosine antibody (ABIN1108460)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: HM-11

Product Details anti-Nitrotyrosine Antibody

Purification: Protein G

Immunogen: Nitrated KLH

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Handling Advice: Keep Sterile, Do Not Add Sodium Azide or any Nitric containing preservative since this will affect the antibody!

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background: Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological? pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage.Synonyms: NO-Tyrosine, Nitro-Tyrosine