SFTPB antibody

Catalog No. ABIN1108991

The Rabbit Polyclonal anti-SFTPB antibody is suitable to detect SFTPB in samples from Human and Pig. It has been validated for WB and EIA.

Quick Overview for SFTPB antibody (ABIN1108991)

Target: SFTPB (Surfactant Protein B (SFTPB))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFTPB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SFTPB Antibody

Specificity: This antibody recognizes the surfactant protein B (SP-B). It rescognizes under nonreducing conditions the most prominent forms of SP-B of 8 kDa and 22 kDa, corresponding to SP-B dimers.

Cross-Reactivity (Details): Species reactivity (tested):Human, pig

Purification: Protein A

Immunogen: Pig SP-B from pig lungs which has been reconstituted in micelles of lysophosphatidylcholine

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for SFTPB

Target: SFTPB (Surfactant Protein B (SFTPB))

Alternative Name: SFTPB

Background: There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behaviour in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS).Synonyms: 18 kDa pulmonary-surfactant protein, Pulmonary surfactant-associated protein B, Pulmonary surfactant-associated proteolipid SPL(Phe), SFTP3, SP-B, Surfactant protein B

Gene ID: 6439

NCBI Accession: NP_000533

UniProt: P07988