PLAU antibody

Catalog No. ABIN1109451

This anti-PLAU antibody is a Mouse Monoclonal antibody detecting PLAU in WB, EIA, IHC (fro), RIA and DB. Suitable for Human.

Quick Overview for PLAU antibody (ABIN1109451)

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAU antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Dot Blot (DB)

Clone: PGM2005

Product Details anti-PLAU Antibody

Specificity: The antibody binds to Human High Molecular Weight single-chain Urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to High Molecular Weight two-chain Urokinase type plasminogen activator (HMW-tcuPA, 52 kDa) and to Low Molecular Weight single-chain Urokinase type plasminogen activator (LMW-scuPA, 33 kDa) with high affinity (Kd= 0.1 nM) and does not inhibit the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is not inhibited. The antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to Urokinase. Crossreacts with Rat scu/tcu PA.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: PEG6000 Precipitation and DEAE Cellulose Chromatography

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL distilled water and add preservative if preferred.

Concentration: 0.2 mg/mL

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for PLAU

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: uPA / PLAU

Background: This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]Synonyms: U-plasminogen activator, Urokinase, Urokinase-type plasminogen activator, Urokinase-type plasminogen activator chain B, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A

Gene ID: 5328

NCBI Accession: NP_001138503

UniProt: P00749

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration