Vimentin antibody

Catalog No. ABIN1109486

The Rat Monoclonal anti-Vimentin antibody has been validated for WB and IF. It is suitable to detect Vimentin in samples from Human, Mouse and Rat.

Quick Overview for Vimentin antibody (ABIN1109486)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: TM71

Product Details anti-Vimentin Antibody

Sequence: CAVLRpSSVP GV

Specificity: This antibody recognizes the site-specific phosphorylation of vimentin at Ser71.

Cross-Reactivity (Details): Species reactivity (tested):Human (U251), mouse (NIH/3T3), rat (3Y1-B)

Purification: Protein G agarose

Immunogen: KLH conjugated phospho-peptide PV71

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM

Gene ID: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis