Vinculin antibody

Catalog No. ABIN1109491

This Mouse Monoclonal antibody specifically detects Vinculin in WB, IF and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Chicken.

Quick Overview for Vinculin antibody (ABIN1109491)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: VIN-54

Product Details anti-Vinculin Antibody

Specificity: This antibody reacts to Vinculin.

Purification: Affinity chromatography

Immunogen: Human vinculin, purified from uterus

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.2 % sodium acetate or neutral PBS.

Concentration: 0,1 mg/mL

Buffer: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Vinculin

Target: Vinculin (VCL)

Background: Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95 % ). Vinculinis mapped to 10q22.1-q23.Synonyms: Metavinculin

Gene ID: 7414

NCBI Accession: NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2