Clathrin Heavy Chain (CLTC) antibody

Catalog No. ABIN7477856

The Rabbit Polyclonal anti-Clathrin Heavy Chain antibody (ABIN7477856) specifically detects Clathrin Heavy Chain in WB, IF and IL. The antibody is reactive with Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum and Actinidia chinensis samples.

Quick Overview for Clathrin Heavy Chain (CLTC) antibody (ABIN7477856)

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum, Actinidia chinensis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunolocalization (IL)

Product Details

Purpose: Anti-Clathrin heavy-chain 1,2

Cross-Reactivity (Details): Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacumAS07 260
Not reactive in: Nicotiana benthamiana

Predicted Reactivity: Amborella trichopoda,Brassica napus,Capsella rubella,Citrus aurantium var. sinensis,Eucalyptus grandis,Glycine max,Chlorella variabilis,Leucaena glauca,Lotus japonicus,Medicago tribuloides,Mimulus guttatus,Musa malaccensis,Oryza sativa,Panicum italicum,Physcomitrium patens,Phaseolus vulgaris,Pisum sativum,Populus balsamifera,Populus trichocarpa,Ricinus communis,Selaginella moellendorffii,Sisymbrium salsugineum,Solanum lycopersicum,Theobroma cacao,Triticum aestivum,Vitis vinifera,Zea mays.

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6

Application Details

Application Notes: 1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)

Comment:

Please, note that fresh samples will provide better restuls (see image below).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin heavy-chain

Background: Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.

Molecular Weight: 193 | 170 kDa (Arabidopsis thaliana)

UniProt: Q0WNJ6