His Tag antibody

Catalog No. ABIN1110327

This anti-His Tag antibody is a Mouse Monoclonal antibody detecting His Tag in WB, ELISA, IP, DB and IL. Suitable for .

Quick Overview for His Tag antibody (ABIN1110327)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunolocalization (IL)

Clone: HIS-H8-EH158

Product Details anti-His Tag Antibody

Sequence: HHHHHH

Cross-Reactivity (Details): 6xHis

Predicted Reactivity: 6xHis

Characteristics: His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies.

Purification: affinity purified

Immunogen: KLH-conjugated synthetic peptide 6xHis

Isotype: IgG2b

Application Details

Application Notes: 1 : 1000 with standard ECL (WB)

Comment:

antibody is present in 10 mM PBS, pH 7.2

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For storage at 4°C for several days to weeks, sodium azide to final 0.1 % can be added. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for His Tag

Target: His Tag

Alternative Name: His-tag

Target Type: Tag

Background: His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies.