beta-2 Microglobulin antibody

Catalog No. ABIN111076

This Mouse Monoclonal antibody specifically detects beta-2 Microglobulin in FACS, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for beta-2 Microglobulin antibody (ABIN111076)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B1G6

Product Details anti-beta-2 Microglobulin Antibody

Specificity: Human beta 2-Microglobulin (MW 12 kDa). Recognizes the HLA-beta 2-Microglobulin complex, free or associated with the membrane. Does not react with other species. The exact epitope for the antibody has not been mapped.

Purification: Protein A Chromatography

Immunogen: Purified human Beta 2-Microglobulin

Isotype: IgG2a

Application Details

Application Notes: Purification of the HLA antigens associated with beta 2-Microglobulin. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry (Frozen Sections or Cell Smears): 1/25-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Concentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS, None, 1 mg/mL BSA

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Following reconstitution store the antibody at -20 °C.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta-2-Microglobulin

Background: Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin

Gene ID: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process