KRT20 antibody

Catalog No. ABIN111169

Product Details anti-KRT20 Antibody

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reactivities on cultured cell lines (tested so far): HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.

Purification: Protein A affinity chromatography

Immunogen: Electrophoretically purified Keratin K20 from human intestinal mucosa

Clone: KS20-10

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen tissue. Immunohistochemistry on paraffin embedded sections (only after after microwavetreatment). Dilute 1/10 with PBS for immunohistochemistry. Incubation time: 1h at RT, orover night at 4C. Western blotting. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Buffer: Final solution contains 0.09 % NaN3, 0.5 % BSA in PBS buffer, pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

References for anti-KRT20 antibody (ABIN111169)

de Wit, van Dalum, Lenferink, Tibbe, Hiltermann, Groen, van Rijn, Terstappen: "The detection of EpCAM(+) and EpCAM(-) circulating tumor cells." in: Scientific reports, Vol. 5, pp. 12270, (2015) (PubMed).

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20 (KRT20 Products)

Synonyms: KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody

Background: Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT

Gene ID: 54474

NCBI Accession: NP_061883

UniProt: P35900