Cytokeratin 18 antibody

Catalog No. ABIN111183

This anti-Cytokeratin 18 antibody is a Mouse Monoclonal antibody detecting Cytokeratin 18 in WB, IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat, Dog, Pig, Cow, Hamster, Sheep, Zebrafish (Danio rerio) and Trout. This Primary Antibody has been cited in 13+ publications.

Quick Overview for Cytokeratin 18 antibody (ABIN111183)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Hamster, Sheep, Zebrafish (Danio rerio), Trout

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Ks18-04

Product Details anti-Cytokeratin 18 Antibody

Specificity: Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish

Purification: Protein A chromatography

Immunogen: Human keratin K18 from HeLa cytoskeletal preparation

Isotype: IgG1

Application Details

Application Notes: Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1.0 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing). Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Store the antiserum at -20 °C (aliquot contents to

References for anti-Cytokeratin 18 antibody (ABIN111183)

Zatloukal, Kufferath, Thueringer, Landegren, Zatloukal, Haybaeck: "Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies." in: PLoS ONE, Vol. 9, Issue 5, pp. e96690, (2014) (PubMed).

Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." in: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, (2012) (PubMed).

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, (2003) (PubMed).

Schaffeld, Knappe, Hunzinger, Markl: "cDNA sequences of the authentic keratins 8 and 18 in zebrafish." in: Differentiation; research in biological diversity, Vol. 71, Issue 1, pp. 73-82, (2003) (PubMed).

Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, (2002) (PubMed).

Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, (2001) (PubMed).

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, (1995) (PubMed).

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18

Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gene ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis