ITGA3 antibody (PE)

Catalog No. ABIN1112174

The Monoclonal anti-ITGA3 antibody has been validated for FACS and IF. It is suitable to detect ITGA3 in samples from Human.

Quick Overview for ITGA3 antibody (PE) (ABIN1112174)

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This ITGA3 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: VJ1-6

Product Details anti-ITGA3 Antibody (PE)

Characteristics: CD49c, a 150kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is expressed by most cell types including T and B lymphocytes

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).

Sample Preparation:

1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD49c PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer.8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: CD49c

Background: The antibody recognizes the VLA-a3 chain of the VLA-3 complex and is reactive with epithelial tissue and basal renal distal tubules.

Pathways: CXCR4-mediated Signaling Events, Integrin Complex