TUBB antibody (C-Term)

Catalog No. ABIN1112713

This Mouse Monoclonal antibody specifically detects TUBB in WB and IF. It exhibits reactivity toward Mouse, Rat, Monkey, Dog and Hamster.

Quick Overview for TUBB antibody (C-Term) (ABIN1112713)

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Mouse, Rat, Monkey, Dog, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-TUBB Antibody

Binding Specificity: C-Term

Specificity: Detect the endogenous levels of total beta-Tubulin protein.

Immunogen: Protein fragment on C-terminal of human beta-Tubulin.

Isotype: IgG1

Application Details

Application Notes: Western Blot (1:5000), Immunofluorescence(1:1000) .Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year. Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin

Background: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of alpha- and beta-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. 1 Mutant huntingtin, disrupted intracellular transport and insulin secretion by direct interference with microtubular beta-tubulin. Mutant huntingtin impaired glucose-stimulated insulin secretion in insulin-producing beta cells, without altering stored levels of insulin. Smith et al. (2009) proposed a novel pathogenetic process by which mutant huntingtin may disrupt hormone exocytosis from beta cells and possibly impair vesicular transport in any cell that expresses the pathogenic protein. 2

Pathways: Microtubule Dynamics, M Phase