WB (1:1000), ICC (1:100-200). Other applications have not been tested. The optimal dilutions should be determined by end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preservative
Sodium azide, Thimerosal (Merthiolate)
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C after thawing (1 week). Aliquot and store at -20 °C for long term (at least one year). Avoid repeated freeze and thaw cycles.
Expiry Date
12 months
Target
Sca-1/Ly-6A/E (Ly6a)
(Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
Alternative Name
Stem cell antigen 1, SCA1
Background
SCA-1(stem cell antigen 1) is a member of the Ly-6 antigen family. It is expressed on testicular side population (SP) cells. The Ly-6 alloantigens have been shown to play a critical role in T lymphocyte activation. The anti-Sca-1 antibody was used in immunohistochemistry experiments to define the structures in several tissues that had previously been shown to contain Ly-6 antigens. In thymus, spleen, and kidney, specific staining of parenchymal cells can be demonstrated, whereas only vasculature reacts with anti-Sca-1 in brain, heart, and liver and possibly in lung.