The Mouse Monoclonal anti-MAP2 antibody has been validated for IHC (fro). It is suitable to detect MAP2 in samples from Human, Mouse, Rat, Cow, Chicken, Quail and Xenopus laevis.
Catalog No. ABIN111293
Quick Overview for MAP2 antibody (FITC) (ABIN111293)
Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain and dendrites and cell bodies of neurons. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c).
Characteristics
Absorption / Emission: 492 nm / 518 nm Molar Ratio: F / P = 3.75
MAP2
Reactivity: Human
ELISA, FACS
Host: Mouse
Monoclonal
5B7
unconjugated
Application Notes
This antibody can be used in immunohistochemistry on tissues or cell usingimmunofluorescence techniques. The suggested working dilution forimmunohistochemical staining is approximately 1: 500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL (OD280)
Buffer
0.01 M PBS, pH 7.2, containing 2 mM EDTA, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
Target
MAP2
(Microtubule-Associated Protein 2 (MAP2))
Alternative Name
MAP-2
Background
Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker