His Tag antibody (HRP)

Catalog No. ABIN1112989

This Mouse antibody specifically detects His Tag in WB. It exhibits reactivity toward .

Quick Overview for His Tag antibody (HRP) (ABIN1112989)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Conjugate: This His Tag antibody is conjugated to HRP

Application: Western Blotting (WB)

Product Details anti-His Tag Antibody (HRP)

Sequence: HHHHHH

Specificity: Detect over-expressed or recombinant proteins containing the 6 × His epitope tag.

Immunogen: A synthetic peptide 6 × His conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: WB (1:5000). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide, Thimerosal (Merthiolate)

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year. Or aliquot and store at -80 °C for long term. Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.