Complement C4A/C4B antibody

Catalog No. ABIN111802

This Mouse Monoclonal antibody specifically detects in WB, EIA, IHC (fro), IHC (p) and IF. It exhibits reactivity toward Human.

Quick Overview for Complement C4A/C4B antibody (ABIN111802)

Target: Complement C4A/C4B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 10-11-14

Product Details

Specificity: This antibody recognises the secreted protein complement component 4d (C4d). C4d was accepted in 2003 into the Banff classification for identification of acute AMR (Racusen et al. 2003). Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection (Sacks and Chowdhury 2002). Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b's short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation (Collins et al. 1999). Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a

Purification: Affinity chromatography on Protein A.

Immunogen: Native human C4 from plasma

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections (1/100-1/750): works very well on acetone-fixed,frozen renal biopsies, Strong staining is observed in the glomeruli and in some cases theperitubular capillaries. Immunohistochemistry on Paraffin Sections: variable result on formalin-fixed,paraffin-embedded sections, It has been observed that pre-treatment with 88 % formic acidfor 20 minutes at room temperature is beneficial. ELISA (1/5000-1/20000). Western Blot. Immunofluorescence (1/250-1/600).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Borate buffered saline, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Complement C4A/C4B

Background: The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ds short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous.Synonyms: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4

Gene ID: 720

NCBI Accession: NP_009224

UniProt: P0C0L4