SFTPD antibody (Lectin Domain) (Biotin)

Catalog No. ABIN111842

The Mouse Monoclonal anti-SFTPD antibody is suitable to detect SFTPD in samples from Human and Rat. It has been validated for WB, IHC (fro) and EIA.

Quick Overview for SFTPD antibody (Lectin Domain) (Biotin) (ABIN111842)

Target: SFTPD (Surfactant Protein D (SFTPD))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFTPD antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: VIF11

Product Details anti-SFTPD Antibody (Biotin)

Binding Specificity: Lectin Domain

Specificity: Antigen Distribution on Tissue Sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.

Purification: Affinity Chromatography

Immunogen: Purified rat surfactant protein D from bronchoalveolar lavage. Remarks: The antigen epitope is localized in the Lectin domain of SP-D.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (IHC) on Frozen sections (2 μg/mL (1/200) for rat cryosections) andParaffin sections. ELISA. Western Blots. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: SFTPD

Background: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

Gene ID: 25350

NCBI Accession: NP_037010

UniProt: P35248