CD209b Antigen (CD209B) antibody (Biotin)

Catalog No. ABIN111862

Product Details

Target: CD209b Antigen (CD209B)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Antigen, Epitope: The antigen is a Glutaraldehyde (0.05 %) resistant protein expressed in the cytoplasm and on the cell surface. Monoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. In combination with MOMA-1 (Cat#SM066P), the murine metallophilic macrophage marker, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages. The antigen recognized by ER-TR9 has recently been shown to be the murine analogue of the human DC-SIGN (Dendritic Cell - Specific ICAM-3 Grabbing Non-Integrin), named SIGN-R1. The antibody reacts with a Mouse subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses. Antigen Distribution Isolated Cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (μ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression.Antigen Distribution

Purification: Affinity Chromatography

Immunogen: Thymus cells.

Clone: ER-TR9

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1.5 μg/mL (1/200). Suggested Positive Control: Mouse spleen). Does not react on routinely processed Paraffin Sections. Has been reported to work in Flow Cytometry (Use 10 μL stock solution to label 10^6 cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (= 0.3 mg/mL Stock Solution).

Concentration: 0.3 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and Following Reconstitution at 2-8 °C. DO NOT FREEZE!

References

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Target Details

Target: CD209b Antigen (CD209B)

Alternative Name: CD209b / DC-SIGNR1 (CD209B Products)

Background: SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7

Gene ID: 69165

NCBI Accession: NP_001032889

UniProt: Q8CJ91