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CD163 antibody

CD163 Reactivity: Human, Monkey WB, FACS, IHC (fro) Host: Mouse Monoclonal RM3-1 unconjugated
Catalog No. ABIN111878
  • Target See all CD163 Antibodies
    CD163
    Reactivity
    • 132
    • 50
    • 30
    • 29
    • 20
    • 5
    • 4
    • 1
    Human, Monkey
    Host
    • 112
    • 44
    • 1
    Mouse
    Clonality
    • 96
    • 60
    • 1
    Monoclonal
    Conjugate
    • 56
    • 18
    • 13
    • 9
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD163 antibody is un-conjugated
    Application
    • 114
    • 71
    • 54
    • 41
    • 27
    • 26
    • 25
    • 24
    • 22
    • 20
    • 15
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody is specific for CD163. Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Monkey. Negative in Pig, Rabbit and Guinea Pig.
    Purification
    Affinity Chromatography
    Immunogen
    Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture
    Clone
    RM3-1
    Isotype
    IgG1
    Top Product
    Discover our top product CD163 Primary Antibody
  • Application Notes
    Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Does not work in routinely processed paraffin sections. Has been described to work in FACS and Western blots. Recommended Positive control: Human placenta.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water to a concentration of 0.4 mg/mL.
    Concentration
    0.4 mg/mL
    Buffer
    Stock solutions contains PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE!
  • Target
    CD163
    Alternative Name
    CD163 (CD163 Products)
    Synonyms
    DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody
    Background
    CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
    Gene ID
    9332
    NCBI Accession
    NP_004235
    UniProt
    Q86VB7
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