ITGA5 antibody (Biotin)

Catalog No. ABIN111906

This anti-ITGA5 antibody is a Mouse Monoclonal antibody detecting ITGA5 in FACS and IHC (fro). Suitable for Human.

Quick Overview for ITGA5 antibody (Biotin) (ABIN111906)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-6

Product Details anti-ITGA5 Antibody (Biotin)

Specificity: This antibody X-6 is useful for the detection of the Fibronectin Receptor, or CD49e. Detects Human granulocytes, monocytes and platelets. Cross reactivity: unknown. X-6 detects an antigen of 154-180 kDa by Immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes, positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.

Cross-Reactivity (Details): Species reactivity (tested):Human: Granulocytes, Monocytes, Platelets.

Purification: Affinity Chromatography

Immunogen: Human Monocytes.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use 10 μL stock solution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e / ITGA5

Background: The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5

Gene ID: 3678

NCBI Accession: NP_002196

UniProt: P08648

Pathways: Integrin Complex