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Desmoglein 2 antibody

DSG2 Reactivity: Human WB, IF, IHC (p), IHC (fro), EIA Host: Mouse Monoclonal 10G11 unconjugated
Catalog No. ABIN112136
  • Target See all Desmoglein 2 (DSG2) Antibodies
    Desmoglein 2 (DSG2)
    Reactivity
    • 89
    • 37
    • 34
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 72
    • 17
    Mouse
    Clonality
    • 63
    • 26
    Monoclonal
    Conjugate
    • 42
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Desmoglein 2 antibody is un-conjugated
    Application
    • 76
    • 26
    • 22
    • 17
    • 16
    • 13
    • 13
    • 12
    • 10
    • 6
    • 5
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    The antibody recognizes Desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide Reacting: Desmoglein 2 (1069aa, calculated Mol. Wt. 116,760). Reactivity on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7. Negative with Mouse and Rat.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
    Clone
    10G11
    Isotype
    IgG1
    Top Product
    Discover our top product DSG2 Primary Antibody
  • Application Notes
    ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Frozen Sections: For better resolution preincubation (directlyafter fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, isrecommended (see Ref.3). Immunohistochemistry on Paraffin Sections: Use microwave treatment. Incubation Time: 1 h at RT for Frozen Tissue and overnight at 2-8 °C for Paraffin Sections. Working Dilution: Dilute 1/10 with PBS, pH 7.4 for Immunohistochemical applications . Recommended Positive Control: CaCo-2, HaCaT.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 1 mL distilled water
    Buffer
    Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Schlegel, Meir, Heupel, Holthöfer, Leube, Waschke: "Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).

  • Target
    Desmoglein 2 (DSG2)
    Alternative Name
    Desmoglein-2 (DSG2 Products)
    Synonyms
    DSG2 antibody, dsc antibody, dsga antibody, si:dkeyp-51f12.6 antibody, wu:fj23f02 antibody, ARVC10 antibody, ARVD10 antibody, CDHF5 antibody, CMD1BB antibody, HDGC antibody, AA408168 antibody, D18Ertd293e antibody, desmoglein 2 antibody, desmoglein 2, tandem duplicate 1 antibody, DSG2 antibody, dsg2.1 antibody, dsg2 antibody, Dsg2 antibody
    Background
    Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.Synonyms: CDHF5, Cadherin family member 5, DSG2, HDGC
    Gene ID
    1829
    NCBI Accession
    NP_001934
    UniProt
    Q14126
    Pathways
    Cell-Cell Junction Organization
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