JUP antibody

Catalog No. ABIN112236

This anti-JUP antibody is a Mouse Monoclonal antibody detecting JUP in WB, IF, IHC (p), IP and IHC (fro). Suitable for Human, Mouse, Rat, Chicken, Cow and Zebrafish (Danio rerio).

Quick Overview for JUP antibody (ABIN112236)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PG 5-1

Product Details anti-JUP Antibody

Specificity: PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells), Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells, adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin). Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat, Mouse, Chicken and Zebrafish.

Purification: Affinity Chromatography on Protein A

Immunogen: Plakoglobin, "band 5" protein from Bovine snout epidermis

Isotype: IgG2b

Application Details

Application Notes: Immunoblotting. Immunoprecipitation. Immunofluorescence Microscopy: 1/5 (1 h at RT). Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water

Buffer: Final solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Junction Plakoglobin

Background: Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.Synonyms: CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP

Gene ID: 3728

NCBI Accession: NP_002221

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location