Desmin antibody

Catalog No. ABIN112305

Product Details anti-Desmin Antibody

Target: Desmin (DES)

Reactivity: Human, Mouse, Rat, Chicken, Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: D9 reacts exclusively with Desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.

Cross-Reactivity (Details): Species reactivity (tested):Chicken, Human, Mouse, Rabbit, Rat, Swine.

Purification: Purified

Immunogen: Desmin isolated from human leiomyoma.

Clone: D9

Isotype: IgG1

Application Details

Application Notes: D9 is suitable for immunoblotting (1: 100 - 1: 1000) and immunohistochemistry on frozenand paraffin embedded tissues (recommended range is 1: 25 - 1: 200 forimmunohistochemistry with avidinbiotinylated horseradish peroxidase complex (ABC) asdetection reagent).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin (DES Products)

Synonyms: DES antibody, des-b antibody, MGC80853 antibody, des antibody, csm1 antibody, csm2 antibody, desm antibody, cmd1i antibody, MGC75911 antibody, LOC100220724 antibody, desmin antibody, cb290 antibody, fb59a12 antibody, wu:fb59a12 antibody, zgc:109859 antibody, CSM1 antibody, CSM2 antibody, LGMD2R antibody, wu:fc11d08 antibody, zgc:154009 antibody, des-a antibody, MGC52614 antibody, desmin antibody, desmin, gene 1 L homeolog antibody, desmin, gene 1 antibody, desmin a antibody, desmin b antibody, desmin, gene 1 S homeolog antibody, desmin, gene 2 S homeolog antibody, DES antibody, des.1.L antibody, des.1 antibody, des antibody, desma antibody, Des antibody, desmb antibody, des.1.S antibody, des.2.S antibody

Background: Desmin (53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.Synonyms: DES

Gene ID: 1674

NCBI Accession: NP_001918

UniProt: P17661