Cytokeratin 13 antibody

Catalog No. ABIN112324

This Mouse Monoclonal antibody specifically detects Cytokeratin 13 in WB, IHC (p), IF and IHC (fro). It exhibits reactivity toward Human, Rat and Cow.

Quick Overview for Cytokeratin 13 antibody (ABIN112324)

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Ks13-1

Product Details anti-Cytokeratin 13 Antibody

Specificity: Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13, with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma, transitional cell carcinoma of the bladder, craniopharyngioma.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine and Rat.

Purification: Protein A affinity chromatography

Immunogen: Keratin K13 of Mr 54.000 purified from human esophagus.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on frozen and paraffin sections: 1: 10, protease pretreatmentimproves the performance of this excellent marker in immunohisto-chemistry: 1/10 withPBS, pH 7.4. Western Blot. Incubation time: 1h at RT, extended with parafin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: Cytokeratin 13

Background: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.Synonyms: CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13

Gene ID: 3860

NCBI Accession: NP_002265

UniProt: P13646