CDH6 antibody (Extracellular Domain)

Catalog No. ABIN112365

The Mouse Monoclonal anti-CDH6 antibody (Clone 2B6) (ABIN112365) specifically detects CDH6 in WB, IHC (p), IHC (fro) and IF. The antibody is reactive with Human samples.

Quick Overview for CDH6 antibody (Extracellular Domain) (ABIN112365)

Target: CDH6 (K-Cadherin (CDH6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDH6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: 2B6

Product Details anti-CDH6 Antibody

Binding Specificity: Extracellular Domain

Specificity: Clone 2B6 recognizes the extracellular domain of Cadherin-6.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Purified

Immunogen: Affinity purified extracellular domain of human cadherin-6-GST fusion protein

Isotype: IgG1

Application Details

Application Notes: Immunoblotting (1: 25 - 1: 250). Immunocytochemistry. Immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mMCaCl2 and 0.1 mM MgCl2. Recommended range is 1: 25 - 1: 50 for immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDH6

Target: CDH6 (K-Cadherin (CDH6))

Alternative Name: Cadherin-6

Background: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.Synonyms: CDH6, Cadherin K, K-Cadherin, Kidney cadherin

Gene ID: 1004

NCBI Accession: NP_004923

UniProt: P55285

Pathways: Cell-Cell Junction Organization