ITGA3 antibody (Isoform 3A, N-Term)

Catalog No. ABIN112369

This Mouse Monoclonal antibody specifically detects ITGA3 in WB, IF and IHC (fro). It exhibits reactivity toward Human and Dog.

Quick Overview for ITGA3 antibody (Isoform 3A, N-Term) (ABIN112369)

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 158A3

Product Details anti-ITGA3 Antibody

Binding Specificity: Isoform 3A, N-Term

Specificity: This antibody Clone 158A3 reacts exclusively with the cytoplasmic domain of non-phosphorylated Integrin subunit α3A. A broad species reactivity is expected because of the conserved nature of the epitope.

Purification: Purified

Immunogen: 158A3 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a synthetic peptide corresponding to the cytoplasmic domain of the integrin subunit a3A including an additional N-terminal cysteine (CRTRALYEAKRQKAEMKSQPSETERLTDDY) coupled to keyhole limpet hemocyanin.

Isotype: IgG2a

Application Details

Application Notes: 158A3 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections. Recommended dilutions: 1/100-1/200 for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: CD49c / ITGA3

Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated a and b subunits. More than 18 a and 8 b subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. For integrin subunits a3 and a6, two cytoplasmic variants, A and B, have been identified.Synonyms: CD49 antigen-like family member C, FRP-2, GAPB3, Galactoprotein B3, ITGA-3, ITGAG3, Integrin alpha-3, MSK18, VLA-3 alpha chain, VLA3

Gene ID: 3675

NCBI Accession: NP_002195

UniProt: P26006

Pathways: CXCR4-mediated Signaling Events, Integrin Complex