ITGA3 antibody (Isoform 3B, N-Term)

Catalog No. ABIN112371

The Mouse Monoclonal anti-ITGA3 antibody (Clone 54B3) (ABIN112371) specifically detects ITGA3 in WB, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for ITGA3 antibody (Isoform 3B, N-Term) (ABIN112371)

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 54B3

Product Details anti-ITGA3 Antibody

Binding Specificity: Isoform 3B, N-Term

Sequence: CTRYYQIMPK YHAVRIREEE RYPPPGSTLP TKK

Specificity: 54B3 recognizes specifically the cytoplasmic domain of integrin subunit a3B which is present in microvascular structures in brain and heart.

Purification: Purified

Immunogen: Synthetic peptide corresponding to a 32 amino acid stretch in the cytoplasmic domain of integrin alpha3B including an appending N-terminal cysteine coupled to keyhole limpet hemocyanin.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting (1: 100 - 1: 1000). Immunocytochemistry. Immunohistochemistry on frozen tissues (1: 25 - 1: 200, with avidin-biotinylated horseradishperoxidase complex (ABC) as detection reagent).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA3

Target: ITGA3 (Integrin, alpha 3 (ITGA3))

Alternative Name: CD49c / ITGA3

Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated α and β subunits. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. For integrin subunits α3 and α6, two cytoplasmic variants, A and B, have been identified.Synonyms: CD49 antigen-like family member C, FRP-2, GAPB3, Galactoprotein B3, ITGA-3, ITGAG3, Integrin alpha-3, MSK18, VLA-3 alpha chain, VLA3

Gene ID: 3675

NCBI Accession: NP_002195

UniProt: P26006

Pathways: CXCR4-mediated Signaling Events, Integrin Complex