Nestin antibody

Catalog No. ABIN112422

This anti-Nestin antibody is a Mouse Monoclonal antibody detecting Nestin in WB, IF, IHC (p) and IHC (fro). Suitable for Mouse and Rat.

Quick Overview for Nestin antibody (ABIN112422)

Target: Nestin (NES)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4D4 (Rat-401)

Product Details anti-Nestin Antibody

Specificity: This antibody recognizes Mouse and Rat Nestin and does not recognizes Human Nestin.

Purification: Ig Fraction

Immunogen: Nestin purified from embryonic rat spinal cord. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Isotype: IgG

Application Details

Application Notes: Western Blot: reacts with a band at 200-220 kDa in reducing gels of newborn rat or mousecell extracts, For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and4.6 % SDS, Membranes should be blocked with milk or BSA, 5 % PAGE gels are suggested. Immunohistochemistry on frozen and paraffin sections: 1/20 - 1/200, We recommendperfusing tissues with 4 % paraformaldehyde at pH 7.4 for light microscopy or with either4 % paraformaldehyde at pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at pH 7.4 for EM, For Immunocytochemistry we recommend using cells fixed in 4 %paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: TRIS buffered saline, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.Synonyms: NES, Nbla00170

Gene ID: 18008

NCBI Accession: NP_057910

UniProt: Q6P5H2