APOA1 antibody

Catalog No. ABIN112523

The Mouse Monoclonal anti-APOA1 antibody is suitable to detect APOA1 in samples from Human. It has been validated for EIA and ELISA (Capture).

Quick Overview for APOA1 antibody (ABIN112523)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: G2

Product Details anti-APOA1 Antibody

Specificity: Reacts with both free Apolipoprotein A1 and HDL bearing Apo-A1. Does not cross-react with ApoE, ApoB or Albumin.Aff. Const.:Kd = 3nM (for Human Apolipoprotein A1)

Purification: Affinity Chromatography on Protein A

Immunogen: Native Human Apolipoprotein A1 from Human plasma.

Isotype: IgG1

Application Details

Application Notes: ELISA (1/2.500 - 1/10.000). This antibody is suitable for coating microtitre plates in asandwich ELISA using catalogue number ABIN114061 for detection. Western Blot (1/250 - 1/1000). Immunohistochemistry on frozen sections (1/20 - 1/80).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.

Buffer: 0.01 M Sodium Phosphate, 0.01 M Sodium Borate, 0.11 M Sodium Chloride with 1 % Mannitol and 1 % Dextran.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitve and should protected from light.

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apolipoprotein A I (APO AI)

Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

Gene ID: 335

NCBI Accession: NP_000030

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism