Borrelia Burgdorferi antibody

Catalog No. ABIN112680

Cited in 6+ publications. The Rabbit Polyclonal anti-Borrelia Burgdorferi antibody (ABIN112680) specifically detects Borrelia Burgdorferi in WB, IF and IHC (p). The antibody is reactive with Borrelia burgdorferi samples.

Quick Overview for Borrelia Burgdorferi antibody (ABIN112680)

Target: Borrelia Burgdorferi

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Borrelia Burgdorferi antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Borrelia Burgdorferi Antibody

Purification: Protein A Chromatography.

Immunogen: Whole cell preparation from B. burgdorferi (Strain: B31 ATCC#35210)

Application Details

Application Notes: Fluorescence Microscopy: IFA specimens should be acetone-fixed for best results. Immunohistochemistry on Formalin Fixed, Paraffin Embedded Sections. Western Blot: Detect bands at 83 kD, 43 kD, 33 kD and 33 kD and additional low MW bands. Also suitable for Conjugation purposes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and without stabilizing proteins.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) -20 °C for longer.

References for anti-Borrelia Burgdorferi antibody (ABIN112680)

Böhringer, Pohlers, Schulze, Albrecht-Eckardt, Piegsa, Weber, Martin, Hünniger, Linde, Guthke, Kurzai: "Candida albicans infection leads to barrier breakdown and a MAPK/NF-κB mediated stress response in the intestinal epithelial cell line C2BBe1." in: Cellular microbiology, Vol. 18, Issue 7, pp. 889-904, (2016) (PubMed).

Hashiguchi, Yoshida, Akashi, Komemoto, Ueda, Ikarashi, Miyauchi, Konno, Yamanaka, Hirose, Kurokawa, Watanabe: "Titanium dioxide nanoparticles exacerbate pneumonia in respiratory syncytial virus (RSV)-infected mice." in: Environmental toxicology and pharmacology, Vol. 39, Issue 2, pp. 879-86, (2016) (PubMed).

Teoh, Johnson, Hanke, McMichael, Jackson: "Blocking development of a CD8+ T cell response by targeting lymphatic recruitment of APC." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2425-31, (2009) (PubMed).

Eisendle, Grabner, Kutzner, Zelger: "Possible role of Borrelia burgdorferi sensu lato infection in lichen sclerosus." in: Archives of dermatology, Vol. 144, Issue 5, pp. 591-8, (2008) (PubMed).

Marques, Hornung, Dally, Philipp: "Detection of immune complexes is not independent of detection of antibodies in Lyme disease patients and does not confirm active infection with Borrelia burgdorferi." in: Clinical and diagnostic laboratory immunology, Vol. 12, Issue 9, pp. 1036-40, (2005) (PubMed).

Zimmer, Bossow, Kolesnikova, Hinz, Neubert, Herrler: "A chimeric respiratory syncytial virus fusion protein functionally replaces the F and HN glycoproteins in recombinant Sendai virus." in: Journal of virology, Vol. 79, Issue 16, pp. 10467-77, (2005) (PubMed).

Target Details for Borrelia Burgdorferi

Target: Borrelia Burgdorferi

Target Type: Bacteria

Background: Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.