CTXB antibody (HRP)

Catalog No. ABIN112703

The Rabbit Polyclonal anti-CTXB antibody is suitable to detect CTXB in samples from Vibrio cholerae. It has been validated for EIA, WB and IHC (fro).

Quick Overview for CTXB antibody (HRP) (ABIN112703)

Target: CTXB (CtxB Gene Product (CTXB))

Reactivity: Vibrio cholerae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTXB antibody is conjugated to HRP

Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CTXB Antibody (HRP)

Specificity: This antibody detects the beta subunit of Cholera toxin.

Immunogen: Purified Choleragenoid.

Application Details

Application Notes: Immunohistochemistry on frozen sections (1: 20 - 1: 200). ELISA (1: 200 - 1: 1000). Western blot (1: 20 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1-2 mg/mL

Buffer: Phosphate buffered saline, pH 7.2, containing 0.002 % Thimerosal and 1 % Bovine serum albumin

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CTXB

Target: CTXB (CtxB Gene Product (CTXB))

Alternative Name: Cholera Enterotoxin Subunit B

Background: The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.Synonyms: Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Choleragenoid, VC_1456, ctxB, toxB

Gene ID: 2613962

NCBI Accession: NP_231099

UniProt: P01556