The Rabbit Polyclonal anti-Listeria Monocytogenes antibody is suitable to detect Listeria Monocytogenes in samples from Listeria monocytogenes. It has been validated for EIA.
Reactivity: Listeria monocytogenes
ELISA, IFA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA. Western blot. Immunoflourescence. Immunohistochemistry on Frozen Sections. Also suitable for conjugation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
4-5 mg/mL (OD280 nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative without stabilizing proteins.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Listeria Monocytogenes
Target Type
Bacteria
Background
The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.