CHRM3 antibody (AA 580-589)

Catalog No. ABIN112905

The Rabbit Polyclonal anti-CHRM3 antibody (ABIN112905) specifically detects CHRM3 in WB and IHC (fro). The antibody is reactive with Human and Rat samples.

Quick Overview for CHRM3 antibody (AA 580-589) (ABIN112905)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CHRM3 Antibody

Binding Specificity: AA 580-589

Specificity: Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.

Immunogen: Synthetic peptide M3 (580-589) covalently attached to a carrier protein.

Application Details

Application Notes: Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.

Preservative: Azide free

Precaution of Use: Do Not Use Sodium Azide as preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: Muscarinic Acetylcholine Receptor M3

Background: This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3

Gene ID: 1131

NCBI Accession: NP_000731

UniProt: P20309

Pathways: Synaptic Membrane