APOB antibody (Biotin)

Catalog No. ABIN112987

This Goat Polyclonal antibody specifically detects APOB in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for APOB antibody (Biotin) (ABIN112987)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-APOB Antibody (Biotin)

Specificity: Binds to Apo B-48/100. No cross reaction with human plasma Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII and Apo E.

Purification: Human LDL-Sepharose Affinity column

Application Details

Application Notes: Can be used for detection of Apo B-100 in plasma and lipoproteins for immunoassay andimmunoblot. Dilution range for immunoblot and ELISA range: 1: 5,000-1: 40,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 10 mM PBS, 0.15 M NaCl, 1 mM EDTA pH 7.2 with 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B (Apo B)

Background: Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 516 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100

Gene ID: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism