APOB antibody (HRP)

Catalog No. ABIN112988

The Goat Polyclonal anti-APOB antibody has been validated for WB and EIA. It is suitable to detect APOB in samples from Human.

Quick Overview for APOB antibody (HRP) (ABIN112988)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-APOB Antibody (HRP)

Specificity: Binds to Human Apo B-48/100. No cross- reaction with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII and Apo E.

Purification: Human Apolipoprotein B-100 Sepharose Affinity Chromatography.

Immunogen: Human LDL.

Application Details

Application Notes: Can be used to detect the existence of Apo B-48/100 in plasma and lipoproteins forImmunoassay and Immunoblot. Dilution range for Immunoblot and ELISA: 2,500-10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.0 mL distilled water. Centrifuge product if not completely clear after standing for 1-2 hours at room temperature.

Concentration: 1.0 mg/mL (prior to lyophilization)

Buffer: 50 mM PBS, 0.1 M Sodium Chloride, pH 7.4 containing 10 mg/mL BSA as stabilizer and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store the antibody undiluted for one month at 2-8 °C or (mix with an equal volume of Glycerol) at -20 °C for longer. Avoid cycles of freezing and thawing.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B (Apo B)

Background: Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/l.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100

Gene ID: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism