APOB antibody

Catalog No. ABIN112989

This anti-APOB antibody is a Goat Polyclonal antibody detecting APOB in WB and EIA. Suitable for Human.

Quick Overview for APOB antibody (ABIN112989)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-APOB Antibody

Specificity: Binds to Human Apo B-48/100. No cross- reaction with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII and Apo E.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Human Apolipoprotein-LDL Sepharose Affinity Chromatography.

Immunogen: Human LDL

Application Details

Application Notes: This Apolipoprotein B-48/100 antibody can be used to detect the existence of ApoB-48/100 in plasma and lipoproteins, for immunoassay (1/10,000-1/80,000),Immunoblotting (1/200-1/10,000), Enzyme Conjugation or Biotinylation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (OD280nm, E0.1% = 1.35)

Buffer: 25 mM PBS, 0.15 M NaCl, pH 7.4 containing 1 mM EDTA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B (Apo B)

Background: Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 516 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.Synonyms: Apo-B, ApoB, ApoB-100, ApoB100

Gene ID: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism