Apo(a) antibody

Catalog No. ABIN113082

The Sheep Polyclonal anti-Apo(a) antibody (ABIN113082) specifically detects Apo(a) in WB and EIA. The antibody is reactive with Mammalian samples.

Quick Overview for Apo(a) antibody (ABIN113082)

Target: Apo(a) (apo(a)) (Apolipoprotein A (apo(a)))

Reactivity: Mammalian

Host: Sheep

Clonality: Polyclonal

Conjugate: This Apo(a) antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Apo(a) Antibody

Specificity: Specifically binds to human Lp(a) and apo(a). No cross-reaction to Apo B-100/48, minor to plasminogen.

Purification: Human Apo (a) Sepharose affinity column. Cross-reactivity to plasminogen was removed by passing plasminogen Sepharose affinity column.

Application Details

Application Notes: Suitable for use in ELISA (1: 7,500-1: 60,000), Western blot and conjugation purposes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL (OD280nm, E0.1% = 1.35)

Buffer: 75 mM PBS, 75 m mM Sodium chloride, pH 7.2 containing 0.02 % Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Apo(a)

Target: Apo(a) (apo(a)) (Apolipoprotein A (apo(a)))

Alternative Name: Apolipoprotein(a) / LP(a)

Background: Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis.Synonyms: Apo(a), LPA

Gene ID: 4018

NCBI Accession: NP_005568

UniProt: P08519