Collagen Type V antibody

Catalog No. ABIN113098

This Rabbit Polyclonal antibody specifically detects Collagen Type V in EIA and IF. It exhibits reactivity toward Rat.

Quick Overview for Collagen Type V antibody (ABIN113098)

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type V antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Product Details anti-Collagen Type V Antibody

Specificity: Cross-Reactivity (% at 1:10 RIA dilution) Rat Collagen Type V 100 % Rat Collagen Types I, III < 0.1 % Rat Elastin < 0.1 %

Purification: Ion exchange chromatography (DEAE-Trisacryl).

Immunogen: Collagen type V extracted and purified from rat skin.

Application Details

Application Notes: Suitable for : ELISA (dilution >1: 100-1: 200). IFA with fluorescein anti-rabbit IgG conjugate (didilution at >1: 40 on frozen rat tissuesections). Immunostaining of extra or intracellular components in light microscopy. Quantitation of Collagen Type V in biological fluids or in culture supernatants. (SP)RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute with 0.5 mL deionized water.

Precaution of Use: Do Not Add Sodium Azide

Handling Advice: . Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20 °C or below.

Target Details for Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: Collagen Type V

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.Synonyms: COL5A1

Gene ID: 85490

NCBI Accession: NP_604447

UniProt: Q9JI03