ACE2 antibody (C-Term)

Catalog No. ABIN113168

The Rabbit Polyclonal anti-ACE2 antibody is suitable to detect ACE2 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for ACE2 antibody (C-Term) (ABIN113168)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACE2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACE2 Antibody

Binding Specificity: AA 788-805, C-Term

Specificity: Reacts with Human Angiotensin Converting Enzyme 2 (ACE2, SARS Receptor) C-terminal, amino acids 788 to 805. Does not crossreact with ACE1.

Purification: Immunoaffinity

Immunogen: Synthetic peptide corresponding to amino acids 788 to 805 of human ACE2 (Genbank accession NP_068576).

Application Details

Application Notes: Suitable for use in Western blot (0.5 to 2 μg/mL). Human kidney or human testis cell lysatecan be used as positive control and a 90 kDa band can be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5mg/mL

Buffer: PBS containing 0.02 % Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background: Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1 7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22.Synonyms: ACE-related carboxypeptidase, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog

Gene ID: 59272

NCBI Accession: NP_068576

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour