Ceruloplasmin antibody

Catalog No. ABIN113276

The Sheep Polyclonal anti-Ceruloplasmin antibody is suitable to detect Ceruloplasmin in samples from Human. It has been validated for EIA and IHC (fro).

Quick Overview for Ceruloplasmin antibody (ABIN113276)

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Ceruloplasmin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Ceruloplasmin Antibody

Specificity: Recognizes Ceruloplasmin. This antibody shows minimal reactivity with related serum proteins.

Purification: Ammonium Sulphate Fractionation

Immunogen: Native human Ceruloplasmin

Application Details

Application Notes: Immunohistochemistry on Frozen Sections (1/20-1/200). ELISA (Has been used at dilutions as high as 1/400K in a simple ELISA with 0.1 μ/wellcoated).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0 mg/mL

Buffer: PBS, pH 7.2 contaioning 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ceruloplasmin

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Alternative Name: Ceruloplasmin

Background: Ceruloplasmin is a copper binding plasma glycoprotein consisting of a single polypeptide chain. It has a molecular weight of 132 kDa and occurs in plasma at a concentration of 150-500 mg/L. Although the physiological functions of Caeruloplasmin are not fully understood it is implicated in a number of possible functions. 95 % of plasma copper is bound to Caeruloplasmin, implying some involvement in copper transport. Caeruloplasmin has been described as both an oxidant and an antioxidant, its exact role or roles in this respect are not clear. Plasma levels of Caeruloplasmin are elevated after inflammation and trauma and it is therefore classified as an acute phase protein. The measurement of Caeruloplasmin in plasma is useful in the diagnosis of Wilson's disease, where levels are greatly reduced. Ceruloplasmin is extremely labile in plasma and rapidly degrades to 115 kDa and 19 kDa fragments. The age of plasma samples should therefore be taken into account when interpreting immunological analysis of ceruloplasmin.Synonyms: CP, Ferroxidase

Gene ID: 1356

NCBI Accession: NP_000087

UniProt: P00450

Pathways: Transition Metal Ion Homeostasis