C3 antibody (HRP)

Catalog No. ABIN113306

The Sheep Polyclonal anti-C3 antibody has been validated for WB and EIA. It is suitable to detect C3 in samples from Human.

Quick Overview for C3 antibody (HRP) (ABIN113306)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This C3 antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-C3 Antibody (HRP)

Specificity: This antibody detects Complement 3c, a polypeptide fragment which is present in C3.

Purification: Affinity chromatography on Protein G.

Immunogen: Complement 3c.

Application Details

Application Notes: Suitable for ELISA (1/200-1/800) and Western blotting (1/500-1/2000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, containing 0.01 % Thiomersal as preservative.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2 - 8 °C. Following reconstitution store at -20 °C.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: C3 plays a central role in the activation of the complement system. Processing of C3 by C3 convertase is the central reaction in both classical and alternative complement pathways, resulting in C3a and C3b. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates, and help to initiate the complement cascade, potentially resulting in cellular apoptosis. C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c and C3dg. Defects in C3 can result in susceptibility to pyogenic infection.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3

Gene ID: 718

NCBI Accession: NP_000055

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling