Collagen Type V antibody

Catalog No. ABIN113975

The Rabbit Polyclonal anti-Collagen Type V antibody has been validated for IHC (fro), IHC (p), EIA, IF and RIA. It is suitable to detect Collagen Type V in samples from Cow, Rat and Mouse.

Quick Overview for Collagen Type V antibody (ABIN113975)

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivity: Cow, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type V antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Radioimmunoassay (RIA)

Product Details anti-Collagen Type V Antibody

Specificity: Rat Collagen type V: 100 % Rat Collagen type I, II and II: < 0.1 % Rat Elastin: < 0.1 % (RIA at 1/100 dilution).

Purification: Ion Exchange Chromatography.

Immunogen: Purified collagen type V from rat skin.

Application Details

Application Notes: RIA. ELISA: > 1/200 (OD > 500). Immunofluorescence: 1/40. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissues: 1/500. Incubation time: 60 min. at RT or 2-8 °C overnight. Positive Control: Rat skin or liver. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA and preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: Collagen Type V

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III.Synonyms: COL5A1

Gene ID: 85490

NCBI Accession: NP_604447

UniProt: Q9JI03