Fibronectin antibody

Catalog No. ABIN113978

The Rabbit Polyclonal anti-Fibronectin antibody has been validated for IHC (p), EIA, IHC (fro) and RIA. It is suitable to detect Fibronectin in samples from Cow.

Quick Overview for Fibronectin antibody (ABIN113978)

Target: Fibronectin

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Purification: Purified

Immunogen: Purified fibronectin from bovine plasma

Application Details

Application Notes: IF: 1: 40. IHC: 1: 200 - 1: 600 for paraffin sections, 60 min at RT or 2-8C over night. ELISA. RIA. Positive control: Bovine skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.

Concentration: App. 1 mg/mL

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

Gene ID: 9913

UniProt: P07589