Fibronectin antibody

Catalog No. ABIN113980

This Rabbit Polyclonal antibody specifically detects Fibronectin in IHC (p), EIA, IHC (fro) and RIA. It exhibits reactivity toward Rat.

Quick Overview for Fibronectin antibody (ABIN113980)

Target: Fibronectin

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Purification: Affinity Chromatography.

Immunogen: Purified Fibronectin from Rat plasma.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 1/200-1/600. Immunofluorescence: 1/80. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Rat Skin, Liver. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Also suitable for use in RIA and ELISA: >/= 1/200 (OD >/=500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA or preservatives.

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumors.Synonyms: CIG, Cold-insoluble globulin, FN1

Gene ID: 10116

UniProt: P04937